This preprint provides insights into the functional dynamics of mitochondria-lipid droplet interactions and highlights potential therapeutic targets for lipid metabolic disorders and non-alcoholic fatty liver disease.
The first paper that introduces Microscoop microscopy, photo-biotinylation and mass spectrometry for unprecedented protein localization and identification.
Microscoop® spatial proteomics enables hypothesis-free proteome identification within submicron structures in FFPE or fresh-frozen tissue sections of brain, intestine and lung tissues (among others) accelerating biomarker discovery and diagnostics and therapeutics development.
This study highlights Microscoop®’s ability to map the proteomic landscape of primary cilia at unprecedented resolution, identifying 4,233 proteins, including 524 known ciliary proteins critical for assembly, transportation, and signaling.
Microscoop® optoproteomics (combined localization and MS) reveals spatially resolved protein complexes such as spliceosomes and histone complexes, highlighting its potential to advance subcellular biology and uncover intricate cellular interactions.
Spatial proteomic discovery at specific subcellular locations often faces challenges due to limitations in current technology. Microscoop® spatial proteomics limitations by enabling hypothesis-free targeted protein identification within individual organelles, allowing exploration of subcellular protein interactions.
